Distinct Reactivity of Transient Receptor Potential Vanilloid Subtype 1 in a Murine Model of Atopic Dermatitis with Serious Scratching
Yan Xia1, Akane Tanaka*, 1, 2, Kumiko Oida1, Akira Matsuda3, Hyosun Jang1, Yosuke Amagai1, Saori Ishizaka1, Hiroshi Matsuda1, 3
Identifiers and Pagination:Year: 2014
First Page: 10
Last Page: 16
Publisher Id: TOALLJ-7-10
Article History:Received Date: 30/4/2014
Revision Received Date: 3/8/2014
Acceptance Date: 1/9/2014
Electronic publication date: 19/11/2014
Collection year: 2014
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abnormality in skin sensitivity may be responsible for unbearable itch in patients with atopic dermatitis (AD).
We evaluated reactivity of NC/Tnd mice, a model for human AD, against various experimental stimulations.
Several behavioral tests were performed after external stimuli were applied to NC/Tnd mice. Transient receptor potential vanilloid subtype 1 (TRPV1) reactivity of neuronal cells collected from the dorsal root ganglions (DRG) was analyzed with a Ca++ influx test. Finally, we evaluated suppressive effect of capsaicin on atopic itch of NC/Tnd mice.
Pain responses to heat, acidic stimulation, and capsaicin injection, which are transduced through TRPV1, were decreased in NC/Tnd mice, when compared to two standard strains BALB/c and C57BL/6 mice. The reactivity of the primary neurons isolated from DRG to capsaicin was markedly reduced in NC/Tnd mice. Topical application of histamine evoked scratching in NC/Tnd mice as well as other two strains; however, the scratching intensities induced by nonhistamine pruritogens were significantly lower in NC/Tnd mice comparing to the two strains. In conventional NC/Tnd mice with AD, topical application of capsaicin reduced the scratching behavior.
TRPV1 is associated with both pain and itch sensation; however, abnormalities in TRPV1 reactivity may involve in severe itch in NC/Tnd mice.